[HELICONIUS] Fwd: HELICONIUS post from durrell@hawaii.edu requires approval

[James Mallet]

Forwarded from Durrell Kapan:
>From:    durrell at hawaii.edu
>Subject: Re: [HELICONIUS] W-linked markers -- sexing young stages of 
>Heliconius
>Date: Wed, 14 Apr 2010 10:03:51 -1000
>
>Hi all,
>
>Larry shared this reference with me a while back. Here are the scans 
>all ready to post on the site.
>
>As you can see, in the females there is a small line that crosses 
>between the last two complete segments.
>
>Durrell

Thanks, Durrell. The mailing list doesn't like attachments. I've put 
the images online as "How to sex Heliconius pupae?" at: 
<http://www.ucl.ac.uk/taxome/jim/Mim2/heliconius_pupa_sex_difference.html>http://www.ucl.ac.uk/taxome/jim/Mim2/heliconius_pupa_sex_difference.html 


Best wishes, Jim

>On Apr 14, 2010, at 5:23 AM, James Mallet wrote:
>
> > It's easy to tell apart the sexes of pupae of most Leps with a 
> bit of practice via morphology and a hand lens, so you don't need 
> genetics or to sample destructively.
> >
> > For Heliconius pupae, there's a nice diagram showing sexual 
> differences of ?Heliconius erato in one of Jocelyn Crane's papers 
> -- would anyone like to put it on the web? I haven't got Crane's 
> papers here, but I think it's probably in this one:
> >
> > Beebe, W., J. Crane, and H. Fleming. 1960. A comparison of eggs, 
> larvae and pupae in fourteen species of Heliconiine butterflies 
> from Trinidad, W.I. Zoologica, New York 45:111-153.
> >
> > I understand it's also possible to tell apart larvae of some 
> Lepidoptera, but I don't think anyone has checked carefully in 
> Heliconius. In small transparent-cuticled leps like tortricids it's 
> possible to see the dark coloured testes  through the cuticle in 
> quite young larvae. Igor Emelianov and I used this in Zeiraphera 
> diniana. (Testes are also dark in Heliconius adults -- not yellow 
> or white as suggested by Alexie for I presume other taxa).
> >
> > Ilik Saccheri claims (pers. comm.) that you can take small 
> amounts of haemolymph from Biston pupae without killing them.
> >
> > Good luck, Jim
> >
> >
> >> On Wed, 2010-04-14 at 11:57 +0100, Jamie Walters wrote:
> >>> Hello all,
> >>>
> >>> I'm curious to know if anyone has had any success (or informative
> >>> failures...?) with developing W-linked markers for Heliconius (or
> >>> any
> >>> butterfly that would be near enough to be useful).
> >>>
> >>> Or, alternatively, if anyone has any particularly clever ideas for
> >>> mining the existing genome data for candidate regions I'd be keen
> >>> to
> >>> hear them.
> >>>
> >>> I'm simply interested in being able to sex juveniles and pupae.
> >>>
> >>>
> >>> Thanks very much,
> >>>
> >>> Jamie
> >>>
> >>> _______________________________________________
> >>> HELICONIUS mailing list
> >>>
> >>> HELICONIUS at ucl.ac.uk
> >>>
> >>> http://www.mailinglists.ucl.ac.uk/mailman/listinfo/heliconius
> >>>
> >>
> >> _______________________________________________
> >> HELICONIUS mailing list
> >> HELICONIUS at ucl.ac.uk
> >> http://www.mailinglists.ucl.ac.uk/mailman/listinfo/heliconius
> > ________________________________
> >
> > James Mallet
> > UCL
> > www.ucl.ac.uk/taxome/jim
> >
> > Helen Putnam Fellow 2009-2010
> > Radcliffe Institute for Advanced Study
> > Harvard University
> > Byerly Hall
> > 8 Garden Street
> > CAMBRIDGE Mass. 02138
> > USA
> >
> > tel: +1-617-496-2780
> > fax: +1-616-495-8136
> >
> > http://www.radcliffe.edu/fellowships/fellows_2010jmallet.aspx
> >
> >
> >
> > _______________________________________________
> > HELICONIUS mailing list
> > HELICONIUS at ucl.ac.uk
> > http://www.mailinglists.ucl.ac.uk/mailman/listinfo/heliconius
>
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________________________________

James Mallet
UCL
www.ucl.ac.uk/taxome/jim

Helen Putnam Fellow 2009-2010
Radcliffe Institute for Advanced Study
Harvard University
Byerly Hall
8 Garden Street
CAMBRIDGE Mass. 02138
USA

tel: +1-617-496-2780
fax: +1-616-495-8136

http://www.radcliffe.edu/fellowships/fellows_2010jmallet.aspx


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