[HELICONIUS] Heliconius genome history

[Jamie Walters]

Dear all,

To clarify, this was the abstract I presented last year at the meeting.  It was indeed where I learned that Jeff Boore was well on his way with monarch along with our friendly and fabulous 'rivals' working on Melitaea.  Indeed this year's meeting should be a very exciting one for lepidopterists.

cheers,

Jamie


James R. Walters, Ph.D.
NSF Post-Doctoral Fellow
Jiggins Group
Zoology Department 
University of Cambridge
jw663 at cam.ac.uk
w: 01223-336644
m: 07544-256000


Begin forwarded message:

> From: James Mallet <j.mallet at ucl.ac.uk>
> Date: January 20, 2011 12:05:15 AM GMT
> To: heliconius at ucl.ac.uk
> Subject: [HELICONIUS] Heliconius genome news
> 
> Published at: http://www.k-state.edu/agc/symp2010/Abstracts-2010.pdf 
> But the research documented here is stated to be "as of April, 2010" -- and considerable advances have happened since. Prepare for more news soon! Danaus and Melitaea genome projects threaten the status of Heliconius as "first butterfly genome."
> ___________________________________________________________________
> 
> 53
> The first butterfly genome? Process and progress in the complete genomic sequencing of Heliconius melpomene, the long-wing passion-flower butterfly.
> 
> *Walters, James R.; Heliconius Genome Consortium
> University of Cambridge, Department of Zoology, Cambridge, United Kingdom
> 
> Heliconius butterflies have been a model system for ecological and evolutionary research for over 150 years. They are known as a Œtextbook‚ example of mimicry where polymorphic wing color patterns within species repeatedly converged between species. Ongoing quantitative, population, and developmental genomic investigations of speciation, wing-coloration, mate choice, and other adaptive phenotypes make Heliconius butterflies a prominent emerging model genomic system.
> 
> Here we report on our strategy and progress in generating a complete genomic sequence for H. melpomene. This sequencing effort was initiated less than 2 years ago and reflects a collaboration between the broader Heliconius genetics research community and the Baylor Human Genome Sequencing Center. The 295 Mbp genome of H. melpomene is being sequenced entirely with Œnext generation‚ technologies, primarily via 454 but with some Solexa data also to be included.
> As of April, 2010 we have generated 6.7 Gbp of 454 sequences which corresponds to ~20x coverage. A Newbler assembly of the data yielded ~14,000 scaffolds with N50 = 62 Kbp and ~105 contigs with N50 = 8.1 Kbp. Ongoing 454 sequencing of an 8 Kbp mate-pair library and additional Solexa runs are expected to substantially improve the assembly in the near future. Scaffolds will be anchored and ordered along the chromosomes via RAD-tag genotyping of a F2 mapping family generated from the sequenced H. melpomene strain. RNA-seq data from several tissues will complement existing Sanger and 454 ESTs from wing tissues to guide genome annotation efforts, which will be conducted in collaboration with EBI‚s Ensembl Genomes initiative.
> 
> ________________________________
> 
> James Mallet
> UCL
> www.ucl.ac.uk/taxome/jim
> 
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